The fluorescence of tyrosyl and tryptophanyl diketopiperazines.
نویسندگان
چکیده
The influence of the peptide group on the quantum yield of phenol and indole has been evaluated from fluorescence measurements on the diketopiperazines of the two chromophores. Strong quenching of both groups occurs in water which is almost completely eliminated in dioxane and dimethyl sulfoxide. Polarization of fluorescence measurements indicate that the diketopiperazines have folded conformations in 95% glycerol solutions. It is suggested that the low yields of tyrosyl and tryptophanyl emission in the native state of proteins may be accounted for, in part, by their interactions with the peptide group.
منابع مشابه
Cyclo(l-tyrosyl-l-tryptophanyl) dimethylformamide solvate
THE STRUCTURE OF THE TITLE COMPOUND [SYSTEMATIC NAME: (3S,6S)-3-(4-hydroxy-benz-yl)-6-(1H-indol-3-ylmeth-yl)piperazine-2,5-dione dimethyl-formamide solvate], C(20)H(19)N(3)O(3)·C(3)H(7)NO, contains hydrogen-bonded tapes typical for diketopiperazines. The structure is stabilized by strong inter-molecular inter-actions of the types O-H⋯O and N-H⋯O involving the dipeptide and the solvent mol-ecule...
متن کاملSubplcosecond Fluorescence Depolarization Studies of Tryptophan and Tryptophanyl Residues of Proteins
Fluorescence depolarization of the exposed tryptophanyl residue in the peptide melittin and the buried tryptophanyl residue in the protein azurin (from the bacteria Pseudomonos aeruginoso) was measured with subpicosecond resolution at various excitation and emission wavelengths. A short time component was found in both the fluorescence decay and fluorescence anisotropy decay for the tryptophany...
متن کاملStudies of the aromatic circular dichroism of Staphylococcal nuclease.
Specific contributions of tyrosyl and of tryptophanyl residues can be distinguished in the near-ultraviolet circular dichroic spectrum of staphylococcal nuclease. Upon binding of the inhibitor deoxythymidine 3',5'-diphosphate in the presence of Ca(++), a significant change in the circular dichroic spectrum results which has been used to characterize the interaction of ligand and enzyme. The dat...
متن کاملStructural Studies on Polypeptide Hormones
The structures of three polypeptide hormones (glucagon, a 25-residue analogue of adrenocorticotropin, and parathyroid hormone) which contain enough residues to permit organization have been evaluated in aqueous solution by fluorescence and circular dichroism. The effects of pH, temperature, and guanidine on both tyrosyl and tryptophanyl emission have been measured. None of the fluorescence para...
متن کاملRational design of an orthogonal tryptophanyl nonsense suppressor tRNA
While a number of aminoacyl tRNA synthetase (aaRS):tRNA pairs have been engineered to alter or expand the genetic code, only the Methanococcus jannaschii tyrosyl tRNA synthetase and tRNA have been used extensively in bacteria, limiting the types and numbers of unnatural amino acids that can be utilized at any one time to expand the genetic code. In order to expand the number and type of aaRS/tR...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 243 22 شماره
صفحات -
تاریخ انتشار 1968